|
| Status |
Public on Apr 26, 2022 |
| Title |
Pt-105 |
| Sample type |
RNA |
| |
|
| Source name |
Formalin-Fixed Paraffin-Embedded (FFPE) tissue
|
| Organism |
Homo sapiens |
| Characteristics |
er.percent: 100
pr.percent: 70
menopausal state: Post
tissue: breast cancer
cdna.conc.: 200.2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
FFPE tissues were stored until the study was performed and four sections of 10 µm thickness of FFPE specimens were obtained for RNA extraction. RNA extraction was performed using the RNeasy FFPE Kit according to the manufacturer’s protocol (Qiagen, Hilden, Germany)).
|
| Label |
biotin
|
| Label protocol |
The extracted RNA was synthesized into cDNA using the Ovation FFPE WTA Systems (Affymetrix, Santa Clara, CA, USA) and the Encore Biotin Module (Affymetrix) kits according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
The procedure was performed using the GeneChip ™ Hybridization, Wash and Stain Kit by Thermo Fisher Scientific.
|
| Scan protocol |
The procedure was performed using the GeneChip ™ Hybridization, Wash and Stain Kit by Thermo Fisher Scientific. The GeneChip Scanner 3000 Dx by Thermo Fisher Scientific was used as the scanner.
|
| Data processing |
This data was normalized by .R/Bioconductor on May 29, 2020
|
| |
|
| Submission date |
Mar 22, 2022 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Takashi Takeshita |
| E-mail(s) |
[email protected]
|
| Organization name |
Kumamoto City Hospital
|
| Street address |
4 Chome-1-60 Higashimachi, Higashi Ward
|
| City |
Kumamoto |
| ZIP/Postal code |
8628505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE199135 |
Clinical Relevance of estrogen reactivity in the breast cancer microenvironment |
|
