|
| Status |
Public on Sep 01, 2011 |
| Title |
231-Empty_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer cell line transduced with eGFP
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: breast cancer cell line MDA-MB-231
ectopic expression: control GFP
rep: 3
|
| Treatment protocol |
MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
|
| Growth protocol |
MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
|
| Label |
biotin
|
| Label protocol |
RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Hybridized to Affymetrix human U133 plus 2.0 GeneChips
|
| Scan protocol |
Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
|
| Description |
Raw data file: Chu_Empty_3_121508.CEL
|
| Data processing |
Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
|
| |
|
| Submission date |
Sep 21, 2010 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Jeffrey E Green |
| E-mail(s) |
[email protected]
|
| Phone |
3014355193
|
| Fax |
3014968709
|
| Organization name |
NCI
|
| Street address |
Rm 4054, 37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE24249 |
GATA3 Reprograms Basal Breast Cancer Cells towards a Luminal Subtype and Inhibits Metastases through Suppression of Lysyl Oxidase |
|
