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Status |
Public on Sep 29, 2010 |
Title |
Patient 12, before month1 IFN-beta injection_chipB |
Sample type |
RNA |
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Source name |
Peripheral blood mononuclear cells
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Organism |
Homo sapiens |
Characteristics |
gender: female age at ifn-beta therapy onset (baseline blood sampling): 28 duration from ms diagnosis to therapy initiation (in months): 1 edss at baseline: 1.5 edss after 1 year: 1.0 edss after 2 years: 2.5 edss after 5 years: 2.5 number of relapses during the year prior to treatment: 1 number of relapses during 1-year follow-up: 0 number of relapses during 2-year follow-up: 1 number of relapses during 5-year follow-up: 2 time from start of therapy to the first relapse (in months): 13 completed years of ifn-beta therapy: >=5
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Treatment protocol |
Patients were treated with subcutaneous IFN-beta-1b (Betaferon, Bayer Healthcare) at standard dose (250 µg every other day).
|
Growth protocol |
Patient blood samples were drawn immediately before first IFN-beta injection as well as two days, one, twelve and twenty-four months post therapy initiation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Blood was separated using isopycnic centrifugation (Ficoll), and peripheral blood mononuclear cells were lysed using chaotropic buffer and cleaned by RNeasy (Qiagen) according to the manufacturers' protocol.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 µg total RNA (Expression Analysis Technical Manual, Affymetrix).
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Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 h at 45°C on Affymetrix HG-U133A and B arrays, respectively. Microarrays were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression data from a multiple sclerosis patient treated with IFN-beta
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Data processing |
The raw probe-level signals were converted to expression values using the MAS5.0 algorithm. Accordingly, probeset specific detection calls (Absent/Present) and changes with respect to baseline (Increase/Decrease/NoChange) were ascertained.
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Submission date |
Sep 29, 2010 |
Last update date |
May 31, 2011 |
Contact name |
Robert Goertsches |
E-mail(s) |
[email protected]
|
Organization name |
University
|
Department |
Neurology
|
Lab |
Neuroimmunology
|
Street address |
Gehlsheimer Strasse 20
|
City |
Rostock |
ZIP/Postal code |
18047 |
Country |
Germany |
|
|
Platform ID |
GPL97 |
Series (1) |
GSE24427 |
Expression data of multiple sclerosis patients receiving subcutaneous Interferon-beta-1b therapy [U133 A and B] |
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