|
| Status |
Public on Mar 01, 2011 |
| Title |
Patient 65N, normal colonic mucosa |
| Sample type |
RNA |
| |
|
| Source name |
normal colonic mucosa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal colonic mucosa
|
| Growth protocol |
Fresh-frozen tissue samples.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent (Invitrogen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 10ug of total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to the Affymetrix HG-U133A chip according to the manufacturer's instructions.
|
| Scan protocol |
The chips were scanned at 570 nm with 3um resolution in an Affymetrix GeneChip scanner (Hewlett Packard).
|
| Description |
65N
Gene expression data from human normal colonic mucosa sample.
|
| Data processing |
The readings from the quantitative scanning were analyzed by the Affymetrix MAS5.0 and normalized using the quantile normalization procedure implemented in robust multiarray analysis (RMA) with GeneSpring software (Silicon Genetics, CA, USA).
|
| |
|
| Submission date |
Oct 04, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Pia Vahteristo |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Helsinki
|
| Street address |
Haartmaninkatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
00014 University of Helsinki |
| Country |
Finland |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE24514 |
Expression data from human MSI colorectal cancer and normal colonic mucosa |
|
