|
| Status |
Public on Apr 25, 2022 |
| Title |
ID8G_2 |
| Sample type |
RNA |
| |
|
| Source name |
Parental_2
|
| Organism |
Mus musculus |
| Characteristics |
cell line: ID8G
|
| Growth protocol |
ID8G and GO2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 4% FBS as well as insulin (5 μg/ml), transferrin (5 μg/ml), and sodium selenite (5 ng/ml) insulin in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| Hybridization protocol |
The Agilent array were hybridized and washed using the manufacturer's protocol.
|
| Scan protocol |
Agilent arrays were scanned using the Agilent Technologies Scanner G2505C.
|
| Data processing |
The data were normalized with 75 Percentile method
|
| |
|
| Submission date |
Apr 22, 2022 |
| Last update date |
Apr 26, 2022 |
| Contact name |
Eiji Sugihara |
| E-mail(s) |
[email protected]
|
| Organization name |
Fujita Health University
|
| Street address |
1-98, Dengakugakubo, Kutsukake-cho,
|
| City |
Toyoake |
| State/province |
Aichi |
| ZIP/Postal code |
4701192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE201275 |
Pigment epithelium–derived factor promotes peritoneal dissemination of ovarian cancer through induction of immunosuppressive macrophages |
|
