strain: Sprague-Dawley tissue: kidney cell type: proximal tubular cells phenotype: type I diabetes treatment: C-peptide
Treatment protocol
Isolated proximal tubular cells from diabetic rats were treated with or without C-peptide for 2 hrs.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from rat proximal tubular cells stored in RNA later using Rneasy (QIAGEN, Valencia, CA, USA).
Label
biotin
Label protocol
Total RNA were amplified and converted to cDNA and labeled using the Whole Transcript (WT) Sense Target Labeling Protocol.
Hybridization protocol
From non-degraded high-quality total RNA, we prepared and hybridized biotinylated complementary ssDNA to Rat Gene 1.0 ST Arrays, and then washed, stained and scanned slides using standard protocols (Affymetrix Inc., Santa Clara, CA).
Scan protocol
We used a GeneChip Scanner 3000 and standard Affymetrix protocols (Affymetrix Inc., Santa Clara, CA).
Description
P50_+CP2
Data processing
Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: PLIER, Background Correction: PM-GCBG, Normalization: Global Median.
