|
| Status |
Public on Oct 20, 2005 |
| Title |
Quiescent Fibroblast Mu11kB- 250 |
| Sample type |
RNA |
| |
|
| Source name |
Quiescent Mock infected Fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
C57/Bl6 MEFs
passage 3
serum starved for 48 hrs.
mock infected for 16 hrs.
|
| Treatment protocol |
Primary wildtype mouse embryo fibroblasts were prepared from 13.5 dpc C57Bl/6 embryos.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin-labeled
|
| Label protocol |
We generated biotin-labeled cDNA by in vitro transcription from the RNA.
|
| |
|
| Hybridization protocol |
The cDNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cDNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cDNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
|
| Scan protocol |
We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
|
| Description |
Quiescent C57/Bl6 fibroblasts were mock infected for 16hrs. RNA was then harvested, transcribed to cDNA, and hybridized to a Mu11kA array.
|
| Data processing |
Imported into genespring and normalized per chip by 50th percentile and by gene by median expression
|
| |
|
| Submission date |
Jun 12, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Zachary Conrad Hartman |
| E-mail(s) |
[email protected]
|
| Phone |
919-684-9197
|
| Organization name |
Duke University
|
| Department |
Surgery
|
| Lab |
Lyerly Lab
|
| Street address |
Research Drive MSRB rm 414
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL76 |
| Series (1) |
| GSE3172 |
Adenovirus infected murine embryonic fibroblasts |
|
