|
| Status |
Public on Oct 15, 2012 |
| Title |
Blood pre EDTA, biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Blood pre challenge with EDTA blood tube
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: EDTA tube blood
tissue: blood
disease state: asthma
treatment: pre-allergen challenge
|
| Treatment protocol |
NA
|
| Growth protocol |
blood were frozen at -80C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kits were used for total RNA extraction from Paxgene blood tube samples; Trizol extraction of total RNA from EDTA blood tube was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridation was performed according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the standard Affymetrix protocol.
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using Partek default analysis settings.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Oct 15, 2012 |
| Contact name |
Scott Tebbutt |
| E-mail(s) |
[email protected]
|
| Organization name |
University of British Columbia
|
| Department |
Medicine
|
| Lab |
James Hogg Research Centre
|
| Street address |
Room 166, 1081 Burrard Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6Z 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE24745 |
Peripheral blood gene expression changes during allergen inhalation challenge in atopic asthmatic individuals |
|
