|
| Status |
Public on Feb 01, 2011 |
| Title |
MBT_TS1_ME2_29_2 |
| Sample type |
RNA |
| |
|
| Source name |
MBT TRANSHETEROZYGOUS TS1/ME2 29ºC
|
| Organism |
Drosophila melanogaster |
| Characteristics |
group: l(3)mbt_TS1_ME2_29
experimentation site: IRB BARCELONA
|
| Treatment protocol |
No additional treatment was used prior to RNA extraction
|
| Growth protocol |
Drosophila larvae were raised in standard Drosophila medium. Immortalised tumours were maintained by serial transplantation into adult hosts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Trizol (Invitrogen). Purity and integrity of the purified RNA was assessed on the Agilent Bioanalyzer 2100. Concentration was determined with a Nanodrop ND-1000 Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
Labeling was carried out following Affymetrix instructions (http://www.affymetrix.com)
|
| |
|
| Hybridization protocol |
Hybridization of the samples to Affymetrix GeneChip® Drosophila Genome 2.0 Array were performed according to the manufacturer’s instructions (http://www.affymetrix.com).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip@ Scanner 3000.
|
| Data processing |
Gene expression data was pre-processed and normalized with R/Bioconductor. The data was quantile normalized, and the summarization was obtained via GC-RMA. In order to remove the bias due to the experimentation site, from all expression values for a given gene we subtracted the average expression of that gene in the wild type samples obtained at the same experimentation site.
|
| |
|
| Submission date |
Oct 25, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
David Rossell |
| E-mail(s) |
[email protected]
|
| Organization name |
IRB Barcelona
|
| Street address |
Baldiri Reixac 10
|
| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE24917 |
Genome wide gene expression profiles of Drosophila l(3)mbt larval brains and cultured tumors |
|
| Relations |
| Reanalyzed by |
GSE119084 |
