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Sample GSM61639 Query DataSets for GSM61639
Status Public on Jun 17, 2005
Title KIV-1-GAS-20B05-1-RML
Sample type RNA
 
Source name Throat Swab
Organisms Streptococcus pyogenes; Macaca fascicularis
Characteristics Day: 9 Gender:Female GAS CFU: 19000
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monkey tonsillar throat swabs with the FastPrep FP 120 kit (Qbiogene, Carlsbad, CA) by immersing the swab tips directly into the FastPrep Blue tubes containing 300 ul 5 mM ammonium aurintricarboxylate, 500 ul CRSR-Blue(Qbiogene), and acid phenol:chloroform (5:1, pH 4.5; Ambion, Austin, TX). The mixture was homogenized (80 sec at speed #5), heated at 65 oC for 20 min, and centrifuged at 16,000xg for 15 min. The aqueous phase was collected, 250 ug glycogen (Roche Applied Science, Indianapolis, IN) and 0.8 ug MS2 carrier RNA (Roche Applied Science, Indianapolis, IN) was added, and the mixture was concentrated to 100 ul with a vacuum concentrator (Eppendorf, Germany). The concentrate was purified using the Qiagen RNeasy 96 kit following the manufacturer's protocol (Qiagen, Valencia, CA).
Label unknown
 
Description Data can be found for this sample in CEL, EXP, and PPA files included as an attachment to this submission. The filename for the CEL and EXP files is the same as the Sample_title field. The column in the PPA file corresponding to this sample is the 20B05 column.
Data processing RMA
 
Submission date Jun 16, 2005
Last update date Jun 22, 2005
Contact name Steve F Porcella
E-mail(s) [email protected]
Phone 406-363-9271
Fax 406-363-9204
Organization name NIAID
Department Rocky Mountain Laboratories
Lab Office of the associate director
Street address 903 SOUTH 4TH Street
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platform ID GPL2129
Series (1)
GSE2713 Longitudinal analysis of the group A Streptococcus transcriptome

Data table header descriptions
ID_REF
VALUE

Data table
ID_REF VALUE
SpM1_ChORF0002_s_at 10.5189
SpM1_ChORF0003_s_at 9.5489
SpM1_ChORF0004_s_at 10.6395
SpM1_ChORF0006_s_at 9.1016
SpM1_ChORF0007_s_at 8.5911
SpM1_ChORF0008_s_at 9.4405
SpM1_ChORF0009_s_at 9.0773
SpM1_ChORF0010_s_at 9.8278
SpM12_ChORF239-12_s_at 9.0986
SpM1_ChORF0013_s_at 8.7774
SpM1_ChORF0015_s_at 8.5256
SpM1_ChORF0016_s_at 8.6329
SpM1_ChORF0019_s_at 9.4657
SpM1_ChORF0020_s_at 8.4952
SpM1_ChORF0021_s_at 10.5715
SpM1_ChORF0022_s_at 9.1138
SpM1_ChORF0023_s_at 10.9040
SpM5_ChORF275f-5_s_at 9.6984
SpM49_ChORF8094-13_s_at 9.3239
SpM1_ChORF0026_s_at 8.8071

Total number of rows: 2620

Table truncated, full table size 70 Kbytes.




Supplementary file Size Download File type/resource
GSM61639.CEL.gz 2.5 Mb (ftp)(http) CEL
GSM61639.EXP.gz 341 b (ftp)(http) EXP

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