|
| Status |
Public on Nov 04, 2010 |
| Title |
ID4+/+TCL1-tg spleen CD19+ B cells collected from 3 mice 3 |
| Sample type |
RNA |
| |
|
| Source name |
ID4+/+TCL1-tg spleen CD19+ B cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: F1 littermate of TCL1 (C3H/B6) and ID4+/+ (CD1) mice
genotype/variation: ID4+/+TCL1-tg
tissue type: spleen
cell type: CD19+ B
age of the mice: 1 month old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from CD19-positive splenic B cells isolated from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice, using the RNEasy column purification Kit (Qiagen).
|
| Label |
enzymatically labeled
|
| Label protocol |
Samples were enzymatically labeled using BioArray HighYield RNA Transcript Labeling kit (Enzo)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, Acetylated BSA (50mg/mL), BioArray Eukaryotic Hybridization Controls (Enzo Life Sciences). Heat 65 degree C for 5 minutes, 99 degree C for 5 minutes, 45 degree C for 5 minutes. Centrifuge top speed for 5 minutes. Hybridize 16 hours at 45 degree C at 60rpm. Fluidics script EukGE-WS2v4_450.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G using Command Console.
|
| Description |
Gene expression data from ID4+/+ TCL1-tg spleen CD19+ B cells
|
| Data processing |
The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
|
| |
|
| Submission date |
Nov 03, 2010 |
| Last update date |
Nov 03, 2010 |
| Contact name |
Lianbo Yu |
| Organization name |
The Ohio State University
|
| Department |
Center for Biostatistics
|
| Lab |
320 Lincoln Tower
|
| Street address |
1800 Cannon Drive
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE25100 |
Expression data from CD19-positive splenic B cells isolated from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice |
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