|
| Status |
Public on Jul 15, 2022 |
| Title |
Glast+ astrocyte_Drd2mGFAP cKO_EAE day17 |
| Sample type |
RNA |
| |
|
| Source name |
Glast+ astrocyte, Drd2mGFAP cKO, EAE day17
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Glast+ astrocyte from spinal cord
strain: C57BL/6
age: 2-3 months
genotype: Drd2mGFAP conditional knockout
condition: EAE day 17
|
| Treatment protocol |
The recipient mice were subcutaneously injected with myelin oligodendrocyte glycoprotein (MOG 35-55, 200 μg/animal) emulsified in CFA containing 5 mg/ml killed Mycobacterium tuberculosis (DIFCO), and intraperitoneally injected with pertussis toxin (400 ng/animal, Sigma-Aldrich) on day 0 and 2. The Glast+ astrocyte from spinal cord was isolated using Miltenyi Biotec Anti-GLAST (ACSA-1) MicroBead Kit following manufacture's recommendations at basal and EAE day17.
|
| Growth protocol |
The mice were maintained on a 12 h light/dark cycle at 23°C with food and water available ad libitum. Animal maintenance and handling were approved by the Institutional Animal Care and Use Committee (IACUC No. NA-004-2016) and were in accordance with the US National Institutes of Health Guide for the Care and Use of Laboratory Animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using Affymetrix WT PLUS Reagent Kit (Cat#902280, Affymetrix, Santa Clara, CA, US) following manufacture's recommendations.
|
| |
|
| Hybridization protocol |
Array hybridization and washing was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US) in a Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US) and a Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
|
| Scan protocol |
Arrays were scanned by Affymetrix GeneChip® Scanner 3000 (Cat#00-00213, Affymetrix, Santa Clara, CA, US).
|
| Description |
Gene expression data from Glast+ astrocyte from spinal cord of Drd2mGFAP cKO mice on EAE day 17
|
| Data processing |
Command Console Software (Affymetrix, Santa Clara, CA, US) was used to control the scanner and summarize probe cell intensity data (CEL file generation) with default settings. Then raw data were normalized by Expression Console.
|
| |
|
| Submission date |
Jun 01, 2022 |
| Last update date |
Jul 15, 2022 |
| Contact name |
Yue Wu |
| E-mail(s) |
[email protected]
|
| Phone |
+86-15821312181
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Neuroscience
|
| Lab |
Jiawei Zhou
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL20775 |
| Series (2) |
| GSE205290 |
Inhibition of astrocytic DRD2 suppresses CNS inflammation in an animal model of multiple sclerosis [astrocyte] |
| GSE205292 |
Inhibition of astrocytic DRD2 suppresses CNS inflammation in an animal model of multiple sclerosis |
|
