|
| Status |
Public on Jul 15, 2022 |
| Title |
spinal cord, control, EAE day 17, biol rep 3 |
| Sample type |
SRA |
| |
|
| Source name |
spinal cord
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spinal cord
strain: C57BL/6
age: 2-3 months
genotype: wt
condition: EAE day 17
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The spinal cord of 2~3-month-old Drd2 cKO mice and littermate control were harvested using Trizol reagent.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
C3_E
|
| Data processing |
The library construction and sequencing was performed by Illumina Hiseq-PE250 at Novogene Corporation. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina following manufacture's recommendations.
Clean data (clean reads) were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data.
Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean reads were aligned to the reference genome using Hisat2 v2.0.5.
Quantification of gene expression level featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Assembly: mm10
Supplementary files format and content: raw_count_table
|
| |
|
| Submission date |
Jun 01, 2022 |
| Last update date |
Jul 15, 2022 |
| Contact name |
Yue Wu |
| E-mail(s) |
[email protected]
|
| Phone |
+86-15821312181
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Neuroscience
|
| Lab |
Jiawei Zhou
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE205291 |
Inhibition of astrocytic DRD2 suppresses CNS inflammation in an animal model of multiple sclerosis [spinal cord] |
| GSE205292 |
Inhibition of astrocytic DRD2 suppresses CNS inflammation in an animal model of multiple sclerosis |
|
| Relations |
| BioSample |
SAMN28813365 |
| SRA |
SRX15557480 |
