|
| Status |
Public on Jul 14, 2022 |
| Title |
20d_rep_2 |
| Sample type |
SRA |
| |
|
| Source name |
Red blood cell in rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: stored 20-day periods with citrate–phosphate–dextrose solution with adenine (CPDA) at 4°C
cell type: Red blood cells
age: 11 weeks
genotype: Wild type
|
| Treatment protocol |
The following 3 experimental conditions were set for rat whole blood depending storage periods: Control (Cont; no storage), 10 days (10d), and 20 days (20d) group.
|
| Growth protocol |
Standard protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted from the purified RBC, using RNAiso Blood (Cat#9112; Takara Bio)
Libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina, and NEBNext rRNA Depletion Kit (Cat# E7770 and E6310; New England Biolabs).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The RNA-Seq reads were aligned to the mRatBN7.2 genome assembly using CLC genomics workbench 22.0 with the default settings.
A statistical differential expression test was used "Differential Expression for RNA-Seq tool" in the CLC software.
Supplementary files format and content: Excel file of statistic test and expression data.
|
| |
|
| Submission date |
Jun 10, 2022 |
| Last update date |
Jul 14, 2022 |
| Contact name |
takehito sugasawa |
| E-mail(s) |
[email protected]
|
| Phone |
+81-29-853-3209
|
| Organization name |
University of tsukuba
|
| Street address |
Ibaraki
|
| City |
Tsukuba |
| State/province |
Select State |
| ZIP/Postal code |
3058577 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20084 |
| Series (1) |
| GSE205806 |
Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion |
|
| Relations |
| BioSample |
SAMN28956620 |
| SRA |
SRX15663427 |
