|
| Status |
Public on Jun 19, 2022 |
| Title |
HBP1R378A1 |
| Sample type |
SRA |
| |
|
| Source name |
Hela
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hela
cell type: cervical cancer cells
genotype: HBP1R378A
treatment: HBP1 Mut
|
| Treatment protocol |
Over expression HBP1 and HBP1R378A in Hela cells,respectively
|
| Growth protocol |
Hela cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Hieff NGS® mRNA Isolation Master Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-T7 |
| |
|
| Data processing |
HISAT2-2.0.4
Sequence reads were trimmed for adaptor sequence/low-quality sequence using HISAT2-2.0.4
Read count extraction and normalization were performed using sequence using HISAT2-2.0.4
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Jun 12, 2022 |
| Last update date |
Jun 19, 2022 |
| Contact name |
ZHANG Tongjia |
| E-mail(s) |
[email protected]
|
| Phone |
18511400217
|
| Organization name |
peking university
|
| Street address |
xueyuan Road 38
|
| City |
beijing |
| ZIP/Postal code |
10010 |
| Country |
China |
| |
|
| Platform ID |
GPL29480 |
| Series (1) |
| GSE205932 |
Methylation of HBP1 by PRMT1 inhibits its tumor-suppressive function through regulating 2 GSN-mediated actin cytoskeleton remodeling |
|
| Relations |
| BioSample |
SAMN29004988 |
| SRA |
SRX15682942 |
