|
| Status |
Public on Jun 27, 2022 |
| Title |
Flu+Flu+MRSA 2 |
| Sample type |
SRA |
| |
|
| Source name |
lung tissue
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cells from bulk lung tissue
strain: WT C57Bl/6
age: 15-18 Wks
treatment: Influenza+Influenza+MRSA
|
| Treatment protocol |
On day 0 mice were given either PBS for the acute group or Influenza (A/X31) for the memory group. 54 days later all mice were given influenza (A/PR/8/34) followed by MRSA (USA300) on day 61.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were euthanized and lungs were taken. Lung tissue was flash frozen with liquid nitrogen, and total RNA was harvested using the Qiagen miRNeasy Mini Kit (cat#217004). After isolation the samples were sequenced using the Illumina TruSeq Platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Reads per sample were collected and mapped to the Mouse Ensembl reference genome (GRCm39) using CLC Genomics Workbench (Qiagen).
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CLC Genomics Workbench (Qiagen).
Assembly: GRCm39
Supplementary files format and content: comma separated value files include RPKM and TPM values for each sample as well as total gene reads
|
| |
|
| Submission date |
Jun 14, 2022 |
| Last update date |
Jun 27, 2022 |
| Contact name |
John Alcorn |
| E-mail(s) |
[email protected]
|
| Organization name |
UPMC Children's Hospital of Pittsburgh
|
| Street address |
4401 Penn Ave
|
| City |
Pittsbrugh |
| State/province |
PA |
| ZIP/Postal code |
15224 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE206095 |
Heterotypic Influenza Infections Mitigate Susceptibility to Secondary Bacterial Infection |
|
| Relations |
| BioSample |
SAMN29042327 |
| SRA |
SRX15699580 |
