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| Status |
Public on Apr 01, 2023 |
| Title |
2_expo_LB30 |
| Sample type |
SRA |
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| Source name |
in vitro, LB 30degreeC
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| Organism |
Bacillus thuringiensis |
| Characteristics |
cell type: in vitro, LB 30degreeC
genotype: WT
growth phase: exponential growth phase OD600nm=1
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| Treatment protocol |
Harvested in RNA-later
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| Growth protocol |
in vitro, LB 30°C or extracted from Galleria mellonella cadavers at 7 days-post-infection
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| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA-Extraction + DNAse treatment
ribosomal RNA depletion (RiboZero) + Truseq Stranded Total RNA prep
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
2_expo_LB30
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| Data processing |
bcl2fastq2-2.18.12 (demultiplex), Cutadapt 1.15 (adapter trimming),
FastQC v0.11.5 (quality control)
mapping (BWA v0.6.2-r126)
FeatureCounts (v1.5.2)
Assembly: GenBank (Assembly: GCA_000306745.1)
Supplementary files format and content: tab-delimited text file including genes and count values for all samples
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| Submission date |
Jun 22, 2022 |
| Last update date |
Apr 01, 2023 |
| Contact name |
Leyla Slamti |
| E-mail(s) |
[email protected]
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| Phone |
+33134652382
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| Organization name |
INRAE
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| Department |
Micalis
|
| Lab |
GME
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| Street address |
Domaine de Vilvert
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| City |
Jouy-en-Josas |
| State/province |
État |
| ZIP/Postal code |
78350 |
| Country |
France |
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| Platform ID |
GPL32378 |
| Series (1) |
| GSE206636 |
A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection |
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| Relations |
| BioSample |
SAMN29246298 |
| SRA |
SRX15823497 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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