|
| Status |
Public on Apr 01, 2023 |
| Title |
3_7j_pi_conv |
| Sample type |
SRA |
| |
|
| Source name |
extracted from insect cadavers at 7dpi
|
| Organism |
Bacillus thuringiensis |
| Characteristics |
cell type: extracted from insect cadavers at 7dpi
genotype: WT
growth phase: 7 dpi
|
| Treatment protocol |
Harvested in RNA-later
|
| Growth protocol |
in vitro, LB 30°C or extracted from Galleria mellonella cadavers at 7 days-post-infection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA-Extraction + DNAse treatment
ribosomal RNA depletion (RiboZero) + Truseq Stranded Total RNA prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
3_7j_pi_conv
|
| Data processing |
bcl2fastq2-2.18.12 (demultiplex), Cutadapt 1.15 (adapter trimming),
FastQC v0.11.5 (quality control)
mapping (BWA v0.6.2-r126)
FeatureCounts (v1.5.2)
Assembly: GenBank (Assembly: GCA_000306745.1)
Supplementary files format and content: tab-delimited text file including genes and count values for all samples
|
| |
|
| Submission date |
Jun 22, 2022 |
| Last update date |
Apr 01, 2023 |
| Contact name |
Leyla Slamti |
| E-mail(s) |
[email protected]
|
| Phone |
+33134652382
|
| Organization name |
INRAE
|
| Department |
Micalis
|
| Lab |
GME
|
| Street address |
Domaine de Vilvert
|
| City |
Jouy-en-Josas |
| State/province |
État |
| ZIP/Postal code |
78350 |
| Country |
France |
| |
|
| Platform ID |
GPL32378 |
| Series (1) |
| GSE206636 |
A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection |
|
| Relations |
| BioSample |
SAMN29246291 |
| SRA |
SRX15823504 |
