|
| Status |
Public on Jul 01, 2022 |
| Title |
overexpression, TET-promoter, IRF1-HA 2 |
| Sample type |
SRA |
| |
|
| Source name |
overexpression
|
| Organism |
Candida albicans |
| Characteristics |
strain background: BWP17 parental strain
genotype: PTET-IRF1-HA
treatment: ChIP antibody: anti-HA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were mechanically opened in standard lysis buffer, with 0.5mm glasbeads during 6 rounds of 45s on a FasPrep at 6.5m speed.
The DNA library was prepared with the TruSeq ChIP library preparation kit from Illumina. 10 ng were used as starting material of each sample. Size selection of the corresponding library was performed by two consecutive AMPure XP bead (Beckman) steps
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Reads were mapped on the available C. albicans genome sequence assembly 22 (candiagenome.org) using Hisat2
binding sites were identified using bPeaks
Assembly: Candida genome databse assembly 22
Supplementary files format and content: location of obtained binding peaks calculated with bPeaks in bedGraph format
|
| |
|
| Submission date |
Jun 27, 2022 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Lasse van Wijlick |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Pasteur
|
| Department |
Mycology
|
| Lab |
BPF
|
| Street address |
25-28 Rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL24725 |
| Series (2) |
| GSE207033 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans [ChIP-Seq] |
| GSE207073 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans |
|
| Relations |
| BioSample |
SAMN29378644 |
| SRA |
SRX15912151 |
