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| Status |
Public on Jul 01, 2022 |
| Title |
2h overexpression, TET-promoter, IRF1, 2 |
| Sample type |
SRA |
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| Source name |
BWP17 parental strain
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| Organism |
Candida albicans |
| Characteristics |
strain: BWP17 parental strain
genotype: PTET-IRF1
treatment: YPD+aTC
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| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were mechanically opened in lysis buffer provided by the RNA extraction kit from Qiagen, with 0.5mm glasbeads during 6 rounds of 45s on a FasPrep at 6.5m speed.
Libraries were prepared with the TruSeq stranded mRNA library kit from Illumina.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
OE_2h_DE.txt
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| Data processing |
Reads were mapped on the available C. albicans genome sequence assembly 22 (candiagenome.org) using Hisat2
DEseq2 was used for differential expression analysis assuming a fold-change of 1.5 with P-adj < 0.05 as significant
Assembly: Candida genome databse assembly 22
Supplementary files format and content: tab-delimited text files include DE values for each Sample
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| Submission date |
Jun 28, 2022 |
| Last update date |
Nov 09, 2022 |
| Contact name |
Lasse van Wijlick |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Pasteur
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| Department |
Mycology
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| Lab |
BPF
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| Street address |
25-28 Rue du Dr Roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platform ID |
GPL19036 |
| Series (2) |
| GSE207072 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans [RNA-Seq] |
| GSE207073 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans |
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| Relations |
| BioSample |
SAMN29397135 |
| SRA |
SRX15927935 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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