|
| Status |
Public on Jul 01, 2022 |
| Title |
3D_carotid atherosclerotic plaque tissue 1 |
| Sample type |
RNA |
| |
|
| Source name |
carotid atherosclerotic plaque tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
tissue: carotid atherosclerotic plaque tissue
|
| Treatment protocol |
Embryos were dechorionated with 50% bleach, put on a cover slip and covered with Halocarbon oil 27 (Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
30 min egg collections of OreR and yw flies at 25C were aged at room temperature (RT) according to the different temporal classes T0-T4.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
lncRNA expression data from carotid atherosclerotic plaque tissue
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 30, 2022 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Qu LeFeng |
| E-mail(s) |
[email protected]
|
| Organization name |
Second Affiliated Hospital of Naval Medical University
|
| Department |
Department of Vascular and Endovascular Surgery
|
| Street address |
Huangpu District Fengyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200003 |
| Country |
China |
| |
|
| Platform ID |
GPL23178 |
| Series (1) |
| GSE207252 |
LncRNA expression in the formation of carotid plaque caused by atherosclerosis |
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