|
| Status |
Public on Oct 14, 2011 |
| Title |
CLL_Peripheral_Blood_GED010 HG_U133plus2 II |
| Sample type |
RNA |
| |
|
| Source name |
peripheral_blood_mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
FISH (del 13q14): 1
FISH (del 13q14 single): 1
FISH (del 11q22-23): 0
FISH (del 17p13): 0
FISH (trisomy 12): 0
FISH (no del 13q14, no del 11q22-23, no del 17p13, no trisomy 12): 0
cell type: peripheral blood mononuclear cell
disease state: CLL
|
| Treatment protocol |
Peripheral blood mononuclear cells from patients with chronic lymphocytic leukaemia (CLL)
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mononuclear cells from peripheral blood (PB) were enriched by Ficoll gradient centrifugation. Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA preparation and in vitro transcription were performed according to standard Affymetrix protocols.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling of antisense cRNA was performed according to standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
Sample hybridisation was performed for 16 hours according to standard Affymetrix protocols
|
| Scan protocol |
Samples were scanned using an Affymetrix GeneChip scanner
|
| Data processing |
Normalization was performed using the Robust Multichip Average (RMA) method. Quality control consisted of visual inspection of the array image for artifacts, assessment of RNA degradation plots, and inspection of rank-vs.-residual plots after normalization and probe set summarization.
|
| |
|
| Submission date |
Nov 23, 2010 |
| Last update date |
Oct 15, 2011 |
| Contact name |
Tobias Herold |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Grosshadern, Ludwig-Maximilians-University (LMU)
|
| Department |
Department of Internal Medicine III
|
| Street address |
Marchioninistr. 15
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE25571 |
Expression analysis of genes located in the minimally deleted regions of 13q14 and 11q22-23 in chronic lymphocytic leukemia – unexpected expression pattern of the RHO GTPase activator ARHGAP20 |
|
| Relations |
| Reanalysis of |
GSM562954 |
