|
| Status |
Public on Nov 09, 2011 |
| Title |
KO_1d-3 |
| Sample type |
RNA |
| |
|
| Source name |
Knockout, 1d
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57/129
genotype: Metfl/fl;Alb-Cre+/- conditional knockout
gender: female
tissue: liver
treatment: CCl4
time: 1 day
|
| Treatment protocol |
8- and 9-week-old female homozygote and control animals (C57/129) were injected i.p. with CCl4 (1ul/g) in mineral oil (Sigma) twice weekly for a period of 4 weeks. Liver samples were collected at various time-points after fibrosis induction using CCL4 ranging from 0 weeks to 3 weeks. Tissue samples were divided into two parts; one was fixed in 10% formalin for histological evaluation and the other was used for RNA analysis.
|
| Growth protocol |
All experiments were conducted according to the National Institutes of Health (NIH) guidelines for animal care and following a study protocol approved by the National Cancer Institute (NCI) Animal Care and Use Committee. Mice had free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen Rneasy Kit. Quality control and measurement were performed using NanoDrop and the Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Totalprep RNA Amplification kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 3 of 4.
|
| Data processing |
Data was processed by using Gene Expression Module v 1.1.1 in Genome Studio V2009.1 from illumina, Inc. The background noise was subtracted and quantile normalization was applied.
|
| |
|
| Submission date |
Nov 23, 2010 |
| Last update date |
Nov 09, 2011 |
| Contact name |
Daekwan Seo |
| E-mail(s) |
[email protected]
|
| Phone |
301-496-5688
|
| Fax |
301-496-0734
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
LEC
|
| Street address |
37 Convent Dr. Rm 4140
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20878 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE25583 |
Loss of c-Met Accelerates Development of Liver Fibrosis in Response to CCl4 Exposure through Deregulation of Multiple Molecular Pathways |
|
