|
| Status |
Public on Nov 29, 2010 |
| Title |
Granta 519CellLine_treated with sox11 siRNA_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Granta 519 cell line, treated with SOX11 siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: mantle cell lymphoma (MCL) cell line
cell line: Granta 519
sirna: treated with SOX11 siRNA
|
| Treatment protocol |
4,000,000 Granta 519 cells were suspended in 100 μl human cell line nucleofector solution C with 100 pmol of either SOX11 siRNA or control siRNA using X-01 electroporation program.
|
| Growth protocol |
Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37 °C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were harvested in 20hs after siRNA transfection. Total RNA from individual transfection experiments was extracted with Qiagen Rneasy Plus Mini Kit. Both the quality and the quantity of RNA were assesed.
|
| Label |
biotin
|
| Label protocol |
Total RNA were amplified and converted to cDNA and labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
| |
|
| Hybridization protocol |
From non-degraded high-quality total RNA we prepared and hybridized biotinylated complementary ssDNA to Human Gene 1.0 ST Arrays, and then washed, stained and scanned slides using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| Scan protocol |
We used standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA)
|
| Description |
BS06_2S
|
| Data processing |
Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: PLIER, Background Correction: PM-GCBG, Normalization: Global Median
|
| |
|
| Submission date |
Nov 26, 2010 |
| Last update date |
Nov 26, 2010 |
| Contact name |
Birgitta Sander |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Street address |
F56 Pathology, Karolinska University Huddinge hospital
|
| City |
Stockholm |
| ZIP/Postal code |
141 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE25621 |
The genes regulated by SOX11 in mantle cell lymphoma |
|
