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Sample GSM63193 Query DataSets for GSM63193
Status Public on Jul 06, 2006
Title E05_30
Sample type RNA
 
Channel 1
Source name WT (BY4700) after 2h shift from M.Gln to M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) after 2h shift from M.Gln to M.Pro
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name gln3∆-gat1∆ (03167b) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics gln3∆-gat1∆ (03167b) on M.Gln
Extracted molecule total RNA
Label Cy5
 
 
Description Scan at low laser power and a PMT gain avoiding saturation
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2600
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R� (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R� (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YBR119W 2240 2030 80 112 113 35 30 31 16 98 88 0 310 316 70 119 122 28 100 100 0 0.429 0.421 0.43 0.401 1.816 0.351 0.576 52 340 273 280 -1.22 82 191 83 197 5886 16415 5.125 6.929 0 0
2 1 2 1 YBR119W 2440 2040 80 119 117 29 30 32 16 100 100 0 307 312 66 129 129 30 98 96 0 0.5 0.475 0.491 0.488 1.834 0.377 0.522 52 340 267 270 -1 89 178 87 183 6102 16201 5.313 6.1 0 0
3 1 3 1 YBR115C 2640 2030 90 1354 1276 303 34 36 22 100 100 0 1174 1143 259 126 128 30 100 100 0 1.26 1.221 1.255 1.218 1.213 1.236 0.937 52 420 2368 2259 0.333 1320 1048 1242 1017 66354 59433 56.364 33.833 0 0
4 1 4 1 YBR115C 2840 2030 90 1139 1101 332 36 41 23 100 100 0 991 1028 265 130 132 31 100 100 0 1.281 1.186 1.21 1.176 1.177 1.194 0.956 52 420 1964 1963 0.357 1103 861 1065 898 57276 53434 46.087 28.903 0 0
5 1 5 1 YBR099C 3060 2050 60 26 28 11 30 34 16 3 0 0 212 208 40 129 129 34 84 71 0 -0.048 -0.025 0.112 0.144 3.733 40.221 0.006 32 176 79 77 null -4 83 -2 79 898 6648 -0.375 2.324 0 0
6 1 6 1 YBR099C 3240 2040 120 26 28 15 28 31 17 12 2 0 158 165 44 120 122 29 65 33 0 -0.053 0 0.211 0.229 4.398 27.668 0.005 120 647 36 45 null -2 38 0 45 3326 19844 -0.176 1.483 -50 0
7 1 7 1 YBR095C 3450 2050 80 76 78 35 30 31 16 78 61 0 386 372 56 120 121 28 100 100 0 0.173 0.19 0.203 0.159 2.435 0.174 0.36 52 340 312 300 -2.532 46 266 48 252 4036 19357 2.938 8.964 0 0
8 1 8 1 YBR095C 3650 2050 80 84 85 24 28 29 15 98 84 0 369 369 57 122 123 30 100 100 0 0.227 0.231 0.231 0.213 1.807 0.211 0.534 52 340 303 304 -2.141 56 247 57 247 4412 19203 3.733 8.2 0 0
9 1 9 1 YBR091C 3850 2060 80 70 75 23 28 32 17 96 67 0 224 222 40 117 118 26 100 90 0 0.393 0.448 0.426 0.432 1.847 0.325 0.305 52 340 149 152 -1.349 42 107 47 105 3922 11536 2.529 4 0 0
10 1 10 1 YBR091C 4050 2070 90 74 75 24 29 31 15 90 69 0 223 217 39 120 121 28 98 82 0 0.437 0.474 0.43 0.434 2.73 0.32 0.302 52 414 148 143 -1.195 45 103 46 97 3876 11308 2.933 3.429 0 0
11 1 11 1 YBR077C 4250 2060 80 79 81 28 26 30 19 88 71 0 326 325 56 121 125 34 100 100 0 0.259 0.27 0.237 0.232 2.051 0.228 0.312 52 340 258 259 -1.952 53 205 55 204 4186 16891 2.684 5.882 0 0
12 1 12 1 YBR077C 4460 2060 90 76 71 25 28 30 15 82 67 0 344 334 64 124 127 33 98 98 0 0.218 0.205 0.218 0.194 1.943 0.19 0.481 52 420 268 253 -2.196 48 220 43 210 3686 17344 2.733 6.273 0 0
13 1 13 1 YBR073W 4660 2060 60 47 51 21 30 31 16 50 21 0 237 234 42 126 127 32 100 84 0 0.153 0.194 0.193 0.19 2.678 0.169 0.131 32 176 128 129 -2.707 17 111 21 108 1646 7480 1.25 3.344 0 0
14 1 14 1 YBR073W 4850 2060 90 51 54 23 26 28 16 63 36 0 198 203 55 124 125 30 80 57 0 0.338 0.354 0.371 0.326 3.627 0.193 0.13 52 420 99 107 -1.566 25 74 28 79 2802 10570 1.625 2.6 0 0
15 1 15 1 YBR069C 5040 2080 70 53 55 19 26 29 14 75 40 0 213 220 51 114 115 28 93 81 0 0.273 0.274 0.259 0.242 2.271 0.189 0.223 32 236 126 135 -1.874 27 99 29 106 1746 7043 1.857 3.75 0 0
16 1 16 1 YBR069C 5250 2090 70 50 52 20 26 27 14 62 40 0 186 192 35 115 118 32 93 56 0 0.338 0.338 0.381 0.346 2.27 0.153 0.089 32 236 95 103 -1.565 24 71 26 77 1676 6144 1.786 2.313 0 0
17 1 17 1 YBR053C 5450 2090 80 53 54 29 22 25 15 67 50 0 255 257 58 118 119 31 98 88 0 0.226 0.23 0.209 0.22 2.557 0.172 0.244 52 340 168 171 -2.144 31 137 32 139 2826 13375 1.933 4.452 0 0
18 1 18 1 YBR053C 5650 2080 80 40 39 16 24 26 14 51 19 0 281 277 47 125 125 29 100 98 0 0.103 0.099 0.126 0.104 2.385 0.067 0.079 52 340 172 167 -3.285 16 156 15 152 2048 14419 0.929 5.241 0 0
19 1 19 1 YBR049C 5860 2080 80 64 68 22 25 27 14 98 71 0 323 325 59 126 127 30 100 98 0 0.198 0.216 0.206 0.2 1.568 0.189 0.513 52 340 236 242 -2.337 39 197 43 199 3528 16878 2.929 6.6 0 0
20 1 20 1 YBR049C 6060 2090 80 78 81 33 24 26 14 90 82 0 324 316 70 124 125 32 98 92 0 0.27 0.297 0.34 0.282 2.365 0.22 0.425 52 334 254 249 -1.889 54 200 57 192 4210 16440 3.929 5.969 0 0

Total number of rows: 15488

Table truncated, full table size 2961 Kbytes.




Supplementary data files not provided
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