|
| Status |
Public on May 25, 2011 |
| Title |
Control for DEX replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
hGR+DEX
|
| Organism |
Xenopus laevis |
| Characteristics |
treatment: hGR with DEX treatment
|
| Treatment protocol |
Animal caps were treated with 30μM DEX in 1x MMR for 4.5 hours before harvesting of total RNA. For condition 1, animal caps were incubated in 1X MMR for 4.5 hours without any treatment
|
| Growth protocol |
1ng of hGR-XEBF3 mRNA and 0.2ng noggin mRNA were co-injected into Xenopus embryos at the one-cell stage. Alternatively, 0.4ng hGR mRNA and 0.2ng noggin mRNA were co-injected in control embryos. At stage 9, animal caps were dissected from the embryo, using either a Gastromaster or a hypodermic needle tip.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from animal caps with the RNeasy mini kit following manufacturer's instructions (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Agilent Two-Color LRILAK Labeling Protocol
|
| |
|
| Channel 2 |
| Source name |
Xebf3-Dex
|
| Organism |
Xenopus laevis |
| Characteristics |
treatment: hGR-Xebf3 without DEX treatment
|
| Treatment protocol |
Animal caps were treated with 30μM DEX in 1x MMR for 4.5 hours before harvesting of total RNA. For condition 1, animal caps were incubated in 1X MMR for 4.5 hours without any treatment
|
| Growth protocol |
1ng of hGR-XEBF3 mRNA and 0.2ng noggin mRNA were co-injected into Xenopus embryos at the one-cell stage. Alternatively, 0.4ng hGR mRNA and 0.2ng noggin mRNA were co-injected in control embryos. At stage 9, animal caps were dissected from the embryo, using either a Gastromaster or a hypodermic needle tip.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from animal caps with the RNeasy mini kit following manufacturer's instructions (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Agilent Two-Color LRILAK Labeling Protocol
|
| |
|
| |
| Hybridization protocol |
Agilent Two-color Gene Expression Hyb/Wash Protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 um resolution.
|
| Description |
Control for DEX
|
| Data processing |
Images where processed using Agilent Feature Extraction software version 9.1.3.1. Lowess normalized log base 10 ratios of Cy3/Cy5 intensity were obtained.
|
| |
|
| Submission date |
Dec 01, 2010 |
| Last update date |
May 25, 2011 |
| Contact name |
Monica Vetter |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Utah
|
| Department |
Neurobiology and Anatomy
|
| Lab |
Monica Vetter
|
| Street address |
50 N. Medical Dr. 517 Wintrobe
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84132 |
| Country |
USA |
| |
|
| Platform ID |
GPL10302 |
| Series (1) |
| GSE25734 |
Transcriptional targets of Xenopus EBF3 in the presence of Noggin : Untreated animal caps vs. Dexamethasone (DEX) treated animal caps |
|
