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Sample GSM63222 Query DataSets for GSM63222
Status Public on Jul 06, 2006
Title E10_80
Sample type RNA
 
Channel 1
Source name WT (BY4700) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name ure2∆ (4709∆URE2) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics ure2∆ (4709∆URE2) on M.Gln
Extracted molecule total RNA
Label Cy5
 
 
Description scan at high laser power and high PMT gain to detect the faintest spots
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2603
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R� (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R� (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YJL214w 1910 750 120 484 508 274 336 377 259 38 13 0 2584 2626 723 2023 2081 602 47 19 0 0.264 0.285 0.333 0.345 3.672 0.058 0.015 120 628 709 775 -1.922 148 561 172 603 60934 315143 0.506 0.905 -50 0
2 1 2 1 YJL214w 2120 750 120 411 450 225 326 362 236 26 7 0 2536 2658 723 1878 1952 565 55 29 0 0.129 0.159 0.217 0.244 4.76 0.05 0.015 120 646 743 904 -2.953 85 658 124 780 53980 318934 0.373 1.25 -50 0
3 1 3 1 aceF 2330 750 90 670 648 248 324 372 252 57 28 0 4487 4656 970 1994 2023 590 100 96 0 0.139 0.122 0.116 0.115 2.262 0.083 0.12 52 420 2839 2986 -2.849 346 2493 324 2662 33684 242109 1.095 4.463 0 0
4 1 4 1 aceF 2550 760 90 774 864 406 330 359 248 71 44 0 4439 4424 759 1832 1882 585 100 100 0 0.17 0.206 0.182 0.17 2.329 0.161 0.208 52 420 3051 3126 -2.554 444 2607 534 2592 44928 230027 2.036 4.345 0 0
5 1 5 1 kdtA 2760 750 90 555 642 353 308 337 274 48 30 0 4470 4478 784 1863 1906 577 100 96 0 0.095 0.128 0.195 0.153 2.643 0.082 0.058 52 420 2854 2949 -3.4 247 2607 334 2615 33368 232869 1.113 4.458 0 0
6 1 6 1 kdtA 2970 750 90 502 566 325 338 379 259 44 15 0 4336 4321 971 1841 1873 550 98 92 0 0.066 0.092 0.151 0.139 2.884 0.051 0.042 52 420 2659 2708 -3.927 164 2495 228 2480 29438 224701 0.722 4.451 0 0
7 1 7 1 tufA 3180 760 90 723 690 340 320 359 258 57 36 0 4544 4652 1233 1808 1844 540 98 96 0 0.147 0.13 0.17 0.156 2.4 0.079 0.125 52 420 3139 3214 -2.763 403 2736 370 2844 35870 241891 1.283 5.2 0 0
8 1 8 1 tufA 3390 760 80 630 656 334 321 362 249 53 32 0 4938 4767 994 1938 1955 575 96 96 0 0.103 0.118 0.145 0.143 2.521 0.091 0.164 52 340 3309 3164 -3.279 309 3000 335 2829 34092 247859 1.181 4.89 0 0
9 1 9 1 buffer 3590 750 120 344 478 647 324 364 264 16 7 0 2012 2068 582 1888 1964 634 22 4 0 0.161 0.856 0.442 0.447 3.653 0.172 0.02 120 661 144 334 -2.632 20 124 154 180 57306 248144 0.432 0.164 -50 0
10 1 10 1 buffer 3810 750 120 311 350 270 362 410 283 14 4 0 1855 1869 645 1881 1932 670 13 3 0 1.962 1 0.375 0.405 3.926 0.032 0.004 120 659 -77 -24 0.972 -51 -26 -12 -12 41986 224325 -0.212 -0.094 -50 0
11 1 11 1 ADN12ngml 4010 750 90 671 747 440 342 394 282 57 38 0 6286 6382 1344 1945 2044 681 100 100 0 0.076 0.091 0.097 0.092 2.6 0.051 0.071 52 414 4670 4842 -3.722 329 4341 405 4437 38834 331879 1.252 6.37 0 0
12 1 12 1 ADN12ngml 4230 760 100 618 691 416 310 359 259 56 30 0 6025 6157 2022 1718 1766 549 100 97 0 0.072 0.086 0.099 0.093 2.398 0.065 0.183 80 552 4615 4820 -3.806 308 4307 381 4439 55256 492550 1.282 7.998 0 0
13 1 13 1 ADN25ngml 4450 760 110 1077 1202 573 288 318 239 92 76 0 12720 12638 3844 1643 1711 571 100 100 0 0.071 0.083 0.075 0.074 1.778 0.078 0.568 80 601 11866 11909 -3.811 789 11077 914 10995 96150 1011022 3.699 19.137 0 0
14 1 14 1 ADN25ngml 4660 760 110 981 1083 495 274 313 229 91 78 0 11218 11514 3473 1598 1620 487 100 100 0 0.073 0.082 0.083 0.082 1.932 0.065 0.386 80 584 10327 10725 -3.766 707 9620 809 9916 86610 921132 3.362 20.316 0 0
15 1 15 1 ADN50ngml 4860 760 100 965 1033 442 256 303 236 93 68 0 11542 11824 3535 1632 1683 541 100 100 0 0.072 0.076 0.082 0.071 1.825 0.064 0.496 80 532 10619 10969 -3.805 709 9910 777 10192 82674 945943 3.093 18.745 0 0
16 1 16 1 ADN50ngml 5070 760 100 1247 1296 585 277 337 250 91 82 0 14442 14367 5338 1687 1722 605 100 100 0 0.076 0.08 0.075 0.079 1.837 0.067 0.547 80 534 13725 13699 -3.717 970 12755 1019 12680 103650 1149331 3.836 20.901 0 0
17 1 17 1 ADN100ngml 5280 760 110 2090 2081 693 285 346 261 100 96 0 27107 27382 4207 1730 1800 647 100 100 0 0.071 0.07 0.068 0.065 1.594 0.067 0.734 80 600 27182 27448 -3.813 1805 25377 1796 25652 166500 2190523 6.648 39.539 0 0
18 1 18 1 ADN100ngml 5500 760 120 1578 1570 694 298 343 245 90 85 0 20783 20552 7549 1774 1833 609 100 98 0 0.067 0.068 0.068 0.065 1.675 0.064 0.727 120 692 20289 20050 -3.892 1280 19009 1272 18778 188354 2466202 5.008 30.737 0 0
19 1 19 1 ADN200ngml 5710 760 110 1630 1679 708 270 327 269 93 87 0 22901 22194 4176 1810 1827 599 100 100 0 0.064 0.069 0.065 0.062 1.743 0.065 0.649 80 580 22451 21793 -3.955 1360 21091 1409 20384 134322 1775489 5.026 34.002 0 0
20 1 20 1 ADN200ngml 5930 770 110 1631 1672 632 266 298 219 100 98 0 21301 21873 4074 1694 1810 636 100 100 0 0.07 0.07 0.068 0.063 1.606 0.067 0.697 80 585 20972 21585 -3.844 1365 19607 1406 20179 133740 1749839 6.274 31.546 0 0

Total number of rows: 12800

Table truncated, full table size 2752 Kbytes.




Supplementary data files not provided

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