|
| Status |
Public on Jun 05, 2023 |
| Title |
HCT116 cells, shControl_1 |
| Sample type |
SRA |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
cell type: Colorectal cancer cell line
genotype: shControl
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by phenol/chloroform.
RNA-Seq was performed using BGISEQ platform (BGI) and Illumina HiSeq .
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Clean reads were aligned to hg38 reference genome with Hisat2 using default parameters. Gene expression levels were counted with HT-seq using default. Differential expression analysis of two groups was performed using the DESeq2 R package.
Assembly: hg38
|
| |
|
| Submission date |
Jul 14, 2022 |
| Last update date |
Jun 05, 2023 |
| Contact name |
sun xuedan |
| E-mail(s) |
[email protected]
|
| Phone |
13339281391
|
| Organization name |
ustc
|
| Street address |
daoxiangcun
|
| City |
hefei |
| ZIP/Postal code |
230022 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (1) |
| GSE208203 |
The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity [RNA-seq] |
|
| Relations |
| BioSample |
SAMN29751963 |
| SRA |
SRX16239635 |
