|
| Status |
Public on May 04, 2011 |
| Title |
Hdh knock-in embryonic stem cells, Q20 line, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/Sv
group: knock-in
genotype (hdh cag repeat length): 7/16
differentiation status: undifferentiated
cell line: Q20
|
| Growth protocol |
Standard growth condition for mouse embryonic stem cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and Qiagen Rneasy mini coulmn clean up
|
| Label |
biotin
|
| Label protocol |
IVT labeling kit from the Affy company
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from Hdh knock-in embryonic stem cells
|
| Data processing |
gcRMA
|
| |
|
| Submission date |
Dec 10, 2010 |
| Last update date |
May 05, 2011 |
| Contact name |
Jong-Min Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Human Genetic Research
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE26001 |
Microarray gene expression data from Hdh knock-out, wild-type and knock-in embryonic stem cells |
|
