|
| Status |
Public on Dec 12, 2011 |
| Title |
heterozygous, prenatal stress, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
left hippocampus of 5HTT +/- prenatal stressed mice
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6
gender: female
tissue: left hippocampus
age: P100
genotype: 5HTT +/-
treatment: prenatal stress
|
| Treatment protocol |
Prenatal maternal stress daily during E13–E17, restraining the dams in transparent 250 ml glass cylinders filled up to a height of 5 mm with water, bright light exposure, 3 times daily, for 45 min. per session. Control pregnant females were left undisturbed in their home cages.
|
| Growth protocol |
heterozygous (+/-) 5-HTT knockout mice ([B6.129(Cg)-Slc6a4tm1Kpl/J] (Bengel, Murphy et al. 1998) were used for breeding. The hippocampi of the offspring were examined.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tissue was homogenized using 500µl PegGOLD RNAPure (Peglab) and metal beads (3 min., 20Hz in a Tissue Lyser (Qiagen)). After that 100µl chloroform was added and centrifuged (5 min., 4°C, 12.000 x g). The water phase was then mixed with 250µl ethanol and from that point on the the protocol of the RNeasy Mini Kit (Qiagen) was followed
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000
|
| Description |
Gene expression data from left hippocampus of mice offspring of 5HTT+/- parents at P100, several behavioural tests
|
| Data processing |
The data were analyzed with Bioconductor packages (under R) and vsn as normalization method using perfect match only
|
| |
|
| Submission date |
Dec 13, 2010 |
| Last update date |
Dec 12, 2011 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
[email protected]
|
| Phone |
+49-931-31 86526
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiologigcal Chemistry
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE26025 |
Sex-specific effects of prenatal stress in 5-Htt deficient mice: towards molecular mechanisms of gene x environment interactions |
|
