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Sample GSM641369 Query DataSets for GSM641369
Status Public on Dec 18, 2010
Title Patient 113, 24 month of REBIF
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics gender: Female
age: 36
cell type: PBMC
individual: Patient 113
treatment: REBIF
time: 24 month of REBIF
disease state: multiple sclerosis
nab status: NAB-
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from fresh peripheral blood mononuclear cells using an RNeasy kit (Quiagen, Santa Clarita, USA)
Label biotin
Label protocol Samples were chemically fragmented and biotinylated using the IVT Labelling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Data processing Chips were processed on an Affymetrix GeneChip Fluidics Station 450. Background adjustment by Robust Multi-Array (RMA) algorithm was applied to probes intensities. Raw probes gene level expression were summarized from the corrected intensities and then quantile normalized.
 
Submission date Dec 16, 2010
Last update date Jun 13, 2012
Contact name Ramil Nurtdinov
E-mail(s) [email protected]
Organization name Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Department Bioinformatics and Genomics
Lab Computational Biology of RNA Processing
Street address Dr. Aiguader 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platform ID GPL570
Series (1)
GSE26104 Search for specific biomarkers of IFN-beta bioactivity in patients with MS

Data table header descriptions
ID_REF
VALUE Log2 RMA signal estimates

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.64
AFFX-BioB-M_at 8.16
AFFX-BioB-3_at 7.67
AFFX-BioC-5_at 9.1
AFFX-BioC-3_at 9.39
AFFX-BioDn-5_at 10.34
AFFX-BioDn-3_at 11.53
AFFX-CreX-5_at 12.53
AFFX-CreX-3_at 12.87
AFFX-DapX-5_at 7.14
AFFX-DapX-M_at 8.38
AFFX-DapX-3_at 9.06
AFFX-LysX-5_at 3.51
AFFX-LysX-M_at 4.84
AFFX-LysX-3_at 6.42
AFFX-PheX-5_at 5.21
AFFX-PheX-M_at 5.72
AFFX-PheX-3_at 6.14
AFFX-ThrX-5_at 5.79
AFFX-ThrX-M_at 6.1

Total number of rows: 54675

Table truncated, full table size 839 Kbytes.




Supplementary file Size Download File type/resource
GSM641369_LF-4711.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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