|
Status |
Public on Dec 18, 2010 |
Title |
Patient 113, 24 month of REBIF |
Sample type |
RNA |
|
|
Source name |
PBMC
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 36 cell type: PBMC individual: Patient 113 treatment: REBIF time: 24 month of REBIF disease state: multiple sclerosis nab status: NAB-
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from fresh peripheral blood mononuclear cells using an RNeasy kit (Quiagen, Santa Clarita, USA)
|
Label |
biotin
|
Label protocol |
Samples were chemically fragmented and biotinylated using the IVT Labelling Kit (Affymetrix).
|
|
|
Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
Data processing |
Chips were processed on an Affymetrix GeneChip Fluidics Station 450. Background adjustment by Robust Multi-Array (RMA) algorithm was applied to probes intensities. Raw probes gene level expression were summarized from the corrected intensities and then quantile normalized.
|
|
|
Submission date |
Dec 16, 2010 |
Last update date |
Jun 13, 2012 |
Contact name |
Ramil Nurtdinov |
E-mail(s) |
[email protected]
|
Organization name |
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
|
Department |
Bioinformatics and Genomics
|
Lab |
Computational Biology of RNA Processing
|
Street address |
Dr. Aiguader 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE26104 |
Search for specific biomarkers of IFN-beta bioactivity in patients with MS |
|