|
| Status |
Public on Mar 01, 2011 |
| Title |
Genotype: PolyQ Atro A66QC, Day: 0, Repl: 3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult flies expressing PolyQ Atro A66QC as they are switched from 18 C to 29 C for ageing
|
| Organism |
Drosophila melanogaster |
| Characteristics |
ageing at 29 c in days: 0
genotype: PolyQ Atro A66QC transgene expressed
|
| Treatment protocol |
Flies were anesthetised with CO2, decapitated and heads were immediately snap frozen in liquid N2.
|
| Growth protocol |
Flies were allowed to develop at 18°C, and newly eclosed flies (0-48 hours) were collected and sacrificed immediately (0 days) or aged for 2 or 14 days at 29°C. The fly food used was standard cornmeal supplemented with yeast and molass. For ageing flies were flipped in fresh vials every 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 20 fly heads using the RNAgents kit (Promega) according to manifacturer instruction; RNA quantity and quality were assessed by Nanodrop 1000 (Thermo Scientific)
|
| Label |
biotin
|
| Label protocol |
The affymetrix protocol was followed for labelling 5ug of total RNA.
|
| |
|
| Hybridization protocol |
The resulting cRNA was Hybridysed on GeneChip Drosophila Genome Array 2.0. The Genechips were washed and stained in the Affimetrix fluidic station.
|
| Scan protocol |
GeneChips were scanned at the EMBL Gene Core Facility
|
| Description |
Replicate 3 for gene expression on log2 scale for adult flies expressing PolyQ Atro A66QC at the outset of ageing at 29 C
A66QC-d0-21
|
| Data processing |
The data were normalized using a variance stabilisation protocol on perfect matches only, and summarized using median polish. The algorithms were implemented in Bioconductor 2.8.0, packages vsn (3.16.0) and affy (1.26.1), on a machine running R 2.11.0 compiled for x86_64-apple-darwin9.8.0
|
| |
|
| Submission date |
Dec 21, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Eric Blanc |
| E-mail(s) |
[email protected]
|
| Phone |
0207 848 6532
|
| Organization name |
King's College London
|
| Department |
MRC Centre for Developmental Neurobiology
|
| Street address |
New Hunt's House
|
| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE26246 |
Polyglutamine Atrophin provokes neurodegeneration in Drosophila |
|
| Relations |
| Reanalyzed by |
GSE119084 |
