|
| Status |
Public on Nov 22, 2023 |
| Title |
HUVECs cells Biol Rep 2, NS Control |
| Sample type |
SRA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
cell type: Primary Endothlelial Cell
genotype: NS
treatment: No treatment
time: 60h post transfection
|
| Treatment protocol |
Expression of ANGPTL4 was silenced in HUVECs (60-70% confluent, P4) by using specific sets of siRNAs using RNAiMAX according to manufactures instructions.
36h post trasfections cells were treated with either CoCl2 (100uM) or appropriate vehical control (PBS) for aditional 24h. In another sets of experiment, 36h posttrasfection, cells were treated either XEN445 (10uM) or apropriate vehical control (DMSO) for aditional 24h.
|
| Growth protocol |
HUVECs (Human umbilical vein endothelial cells, P2) were obtained from the Yale School of Medicine, Vascular Biology and Therapeutics Core facility and cultured in M199/EBSS medium supplemented with 20% fetal bovine serum (FBS), 2 mM Glutamine and antibiotics in humidified atmosphere with 5% CO2 at 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries were generated from control cells using TrueSeq Small RNA Library preparation (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
NS-vs-siANG-Basal-Raw-gene-counts.txt
NS-Ctrl2_256_321_S88
|
| Data processing |
Reads aligned to hg19 build of human genome with STAR
Quantified to hr19 RefSeq annotation model through Partek E/M
Genes were normalized as counts per million
Assembly: hg19
Supplementary files format and content: tab-delimited text file include cpm values for each Sample (raw gene count)
|
| |
|
| Submission date |
Aug 12, 2022 |
| Last update date |
Nov 22, 2023 |
| Contact name |
Bal Krishna Chaube |
| E-mail(s) |
[email protected]
|
| Organization name |
Yale University
|
| Department |
Comparative Medicine
|
| Lab |
414K
|
| Street address |
Amistad Research Building, Amistad St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE211128 |
Suppression of ANGPTL4 Reprograms Endothelial Cell Metabolism and Inhibits Angiogenesis |
|
| Relations |
| BioSample |
SAMN30282181 |
| SRA |
SRX17041551 |
