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Sample GSM647247 Query DataSets for GSM647247
Status Public on Dec 31, 2010
Title Liver, Control (sterilized distilled water administrated for 4 weeks) #2
Sample type RNA
 
Source name Liver, administrated control sterilized distilled water for 4 weeks
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley (SD)
gender: male
age: 8 weeks
tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using ISOGEN (Nippon Gene Co., Ltd., Toyama, Japan), then purified with an RNeasy mini kit (Qiagen K.K., Tokyo, Japan).
Label Biotin
Label protocol Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 2 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 30, 2010
Last update date Dec 31, 2010
Contact name Yuji Nakai
Organization name Hirosaki University
Department Institute of Regional Innovation
Lab Section of Food Sciences
Street address 2-1-1, Yanagawa
City Aomori
State/province Aomori
ZIP/Postal code 038-0012
Country Japan
 
Platform ID GPL1355
Series (1)
GSE26363 Hepatic gene expression profile in hydrogen water administrated SD rat

Data table header descriptions
ID_REF
VALUE MAS5.0 transformed signal
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 61.0692 P 0.000445901
AFFX-BioB-M_at 139.047 P 7.00668e-05
AFFX-BioB-3_at 66.9352 P 9.4506e-05
AFFX-BioC-5_at 287.28 P 4.42873e-05
AFFX-BioC-3_at 278.182 P 4.42873e-05
AFFX-BioDn-5_at 573.931 P 9.4506e-05
AFFX-BioDn-3_at 1121.4 P 4.42873e-05
AFFX-CreX-5_at 3442.71 P 4.42873e-05
AFFX-CreX-3_at 4086.92 P 4.42873e-05
AFFX-DapX-5_at 1.84811 A 0.631573
AFFX-DapX-M_at 4.0769 A 0.52976
AFFX-DapX-3_at 0.205873 A 0.978098
AFFX-LysX-5_at 3.93175 A 0.28771
AFFX-LysX-M_at 1.51612 A 0.5
AFFX-LysX-3_at 9.10411 M 0.054198
AFFX-PheX-5_at 0.366586 A 0.910522
AFFX-PheX-M_at 0.583193 A 0.941556
AFFX-PheX-3_at 1.90979 A 0.783476
AFFX-ThrX-5_at 0.476969 A 0.883908
AFFX-ThrX-M_at 2.40908 A 0.470241

Total number of rows: 31099

Table truncated, full table size 950 Kbytes.




Supplementary file Size Download File type/resource
GSM647247.CEL.gz 2.3 Mb (ftp)(http) CEL
GSM647247.CHP.gz 170.9 Kb (ftp)(http) CHP
Processed data included within Sample table

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