|
| Status |
Public on Jan 13, 2011 |
| Title |
Control_biological rep17 |
| Sample type |
RNA |
| |
|
| Source name |
Children ≤ 10 years old
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Whole Blood
disease state: normal control
age (years): 2.9
outcome: Survivor
|
| Treatment protocol |
standard ICU care for septic shock
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen extraction from PaxGene whole blood RNA collection tubes
|
| Label |
biotin
|
| Label protocol |
WT-Ovation Pico (NuGen)
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from normal control
|
| Data processing |
Gene expression was measured using one patient sample per chip. Image files were captured using an Affymetrix GeneChip Scanner 3000. CEL files were subsequently preprocessed using Robust Multiple-Array Average (RMA) normalization and GeneSpring GX 7.3 software (Agilent Technologies, Palo Alto, CA). All signal intensity-based data were used after RMA normalization, which specifically suppresses all but significant variation among lower intensity probe sets. All chips representing patient samples were then normalized to the respective median values of controls.
|
| |
|
| Submission date |
Dec 30, 2010 |
| Last update date |
Jan 13, 2011 |
| Contact name |
Hector R Wong |
| E-mail(s) |
[email protected]
|
| Phone |
513-636-4259
|
| Fax |
513-636-4267
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Pediatrics
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26378 |
Expression data from validation cohort of children with septic shock |
|
