|
| Status |
Public on Jan 02, 2012 |
| Title |
control, VP lobe, rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
mouse prostate tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
prostate tissue: VP lobe
group: control
|
| Treatment protocol |
Control group: 5 C57BL/6J mice, 8-weeks of age fed with control diet ad libitum for 12 weeks. Experimental group: 5 C57BL/6J mice, 8-weeks of age fed with ad libitum high fat diet for 12 weeks.
|
| Growth protocol |
After 12-weeks, the ventral (VP), dorsolateral (DLP) and anterior (AP) prostate lobes from control group and the group fed with high fat diet were removed and placed in RNase-free PBS and snapped-frozen in liquid nitrogen and stored at -70 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from VP and DLP lobes of mice.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix Mouse 430 2.0 chips for 16 hours.
|
| Scan protocol |
Affymetrix GeneChip scanner
|
| Description |
mouse prostate
|
| Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
|
| |
|
| Submission date |
Jan 05, 2011 |
| Last update date |
Jan 02, 2012 |
| Contact name |
Yoshitaka Sekine |
| E-mail(s) |
[email protected]
|
| Organization name |
NHLBI
|
| Street address |
10 Center Dr
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE26464 |
High Fat Diet Reduces the Expression of Glutathione Peroxidase 3 in Mouse Prostate |
|
