cell line: Huh7 cell type: liver cancer sirna: CSN5
Treatment protocol
15 nM siRNA for 48h.
Growth protocol
DMEM/F - 12 (+ 10% fetal bovine serum).
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with the Qiagen RNeasy Kit. Quality control and measurement were performed using NanoDrop and Agilent Bioanalyzer.
Label
biotin
Label protocol
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification kit for Illumina arrays.
Hybridization protocol
Standard Illumina hybridization protocol.
Scan protocol
Standard Illumina scanning protocol.
Description
Replicate 2 of 4.
Data processing
Data was processed by using Gene Expression Module v 1.1.1 in Genome Studio V2009.1 from illumina, Inc. The background noise was subtracted and quantile normalization was applied.
