|
| Status |
Public on May 23, 2011 |
| Title |
3xFlag-HoxB4_Promoter 1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Input
|
| Organism |
Mus musculus |
| Characteristics |
cell line: ES cell line B6N-22UTR
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromain immunoprecipitation (ChIP) was carried out as previously described (Endoh et al., 2008). Purified immunoprecipitated and input DNA was subjected to T7 RNA polymerase-based amplification as described previously (van Bakel et al., 2008).
|
| Label |
Cy3
|
| Label protocol |
Agilent mammalian ChIP-on-chip protocol (ver.9.0)
|
| |
|
| Channel 2 |
| Source name |
Flag-IP
|
| Organism |
Mus musculus |
| Characteristics |
cell line: ES cell line B6N-22UTR
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromain immunoprecipitation (ChIP) was carried out as previously described (Endoh et al., 2008). Purified immunoprecipitated and input DNA was subjected to T7 RNA polymerase-based amplification as described previously (van Bakel et al., 2008).
|
| Label |
Cy5
|
| Label protocol |
Agilent mammalian ChIP-on-chip protocol (ver.9.0)
|
| |
|
| |
| Hybridization protocol |
Agilent mammalian ChIP-on-chip protocol (ver.9.0)
|
| Scan protocol |
Scanned on an Agilent DNA Microarray Scanner G2505C using Agilent Scan Control software Ver. A.8.4.1 (Protocol: ChIP-v1_10_Apr08).
|
| Description |
ChIP-on-chip data from mouse ES cells-derived hematopoietic progenitor cells overexpressing 3xFLAG-HoxB4
|
| Data processing |
Scanned images were quantified with Agilent Feature Extraction software under standard conditions.
|
| |
|
| Submission date |
Jan 14, 2011 |
| Last update date |
May 23, 2011 |
| Contact name |
Takaho A. Endo |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Laboratory for Integrative Genomics
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4128 |
| Series (1) |
| GSE26653 |
HoxB4 in hematopoietic stem cells |
|
