|
| Status |
Public on Sep 16, 2022 |
| Title |
D071 (IPF control) |
| Sample type |
SRA |
| |
|
| Source name |
Normal Donor lung 24 years old
|
| Organism |
Homo sapiens |
| Characteristics |
number: n=1
tissue: lung
sample type: Control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions were processed through the 10X Chromium to generate single-cell cDNA libraries attached to microbeads
Microbeads were counted, and amplified by PCR, and the 3' end of the cDNA was prepared for sequencing using a modified Nextera XT protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x 3' v2 scRNA-seq
|
| Data processing |
Read alignment and gene-expression quantification of mouse single-cell RNA-seq (scRNA-seq) were performed using Cell Ranger version 2.1.1 pipeline (10x Genomics).
For scRNA-seq alignment and quantification, Cell Ranger pre-built mouse (hg19) reference package was used.
Assembly: hg19
Supplementary files format and content: Raw Feature Barcode Matrix and 10x Cell Ranger filtered Feature Barcode Matirx in HDF5 format
|
| |
|
| Submission date |
Sep 09, 2022 |
| Last update date |
Sep 16, 2022 |
| Contact name |
Yan Xu |
| E-mail(s) |
[email protected]
|
| Phone |
513-6368921
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE213017 |
Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling (Human). |
| GSE213018 |
Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling |
|
