|
| Status |
Public on Sep 21, 2005 |
| Title |
HSC_collagen_1 |
| Sample type |
RNA |
| |
|
| Source name |
cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
CD34+ cells isolated from umbilical cord blood, cultured in collagen I gels
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit
|
| Label |
Biotin/Streptavidin Phycoerythrin
|
| Label protocol |
Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II, starting the first amplification cycle with 100ng total RNA
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol for eukaryotic samples
|
| Scan protocol |
Affymetrix standard procedure
|
| Description |
further details in Oswald et al. "Gene expression profiling of CD34+ hematopoietic cells expanded in a collagen I matrix"
|
| Data processing |
Affymetrix MAS 5.0, global scaling strategy, TGT 500
|
| |
|
| Submission date |
Jul 26, 2005 |
| Last update date |
Sep 21, 2005 |
| Contact name |
Martin Bornhaeuser |
| E-mail(s) |
[email protected]
|
| Phone |
++49-351-458-4704
|
| Fax |
++49-351-458-5389
|
| Organization name |
Universitätsklinikum Carl Gustav Carus
|
| Department |
Med. Klinik und Poliklinik
|
| Street address |
Fetscherstrasse 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE3003 |
Gene expression profiling of CD34+ hematopoietic cells expanded in a collagen I matrix |
|
