|
| Status |
Public on Dec 20, 2011 |
| Title |
Yorkie overexpressing discs, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila pupal eye discs of GMR Gal4, UAS Yki genotype
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Pupal Eye Discs
genotype: GMR Gal4 crossed to UAS Yki
age: 40 hrs after pupariation
|
| Treatment protocol |
The pupae were dissected in PBS
|
| Growth protocol |
The genotypes were grown at 29 deg C until about 40 hrs after pupariation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Qiagen RNAeasy kit)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3G scanner
|
| Description |
Gene expression data from GMR Gal4, UAS Yki pupal eye discs
|
| Data processing |
The data were analyzed with Expression console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shubha Gururaja Rao |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Department |
MCDB
|
| Lab |
Banerjee Lab
|
| Street address |
5032 Terasaki Life Sciences Building, 621 Charles E. Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90034 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE26700 |
Expression data from Control and Yorkie overexpression pupal eye discs |
|
| Relations |
| Reanalyzed by |
GSE119084 |
