|
| Status |
Public on Feb 16, 2006 |
| Title |
KIU_127B00 |
| Sample type |
RNA |
| |
|
| Source name |
breast
|
| Organism |
Homo sapiens |
| Characteristics |
sample name: KIU_127B00
node: 0
size: 2.2
grade: 3
ggi: 0.679034
age: 57
er: 1
time rfs: 699
distant rfs: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturer's instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturer's instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)
|
| Label |
biotin
|
| Label protocol |
Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Tumor samples from untreated primary breast cancer patients.
|
| Data processing |
RMA Bioconductor
|
| |
|
| Submission date |
Jul 26, 2005 |
| Last update date |
May 31, 2013 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
[email protected]
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE2990 |
Gene Expression Profiling in Breast Cancer: Understanding the Molecular Basis of Histologic Grade To Improve Prognosis |
| GSE6532 |
Definition of clinically distinct molecular subtypes in estrogen receptor positive breast carcinomas using genomic grade |
|
| Relations |
| Reanalyzed by |
GSE47561 |
