|
| Status |
Public on Jul 21, 2011 |
| Title |
female_restricted_diet_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
female head/thorax, restricted diet
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: female
tissue: head/thorax
genetic background: canton-s
diet: restricted
age: 10 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from at least 75 females using Trizol (Invitrogen) and further purified using RNeasy columns (QIAGEN). 5 μg total RNA was used with Affymetrix One Cycle DNA conversion Kit (Cat # 900431) and all steps were carried out according to the Affymetrix manual
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
The arrays are hybed overnight (16 hr) at 45C 60 rpm. Fluidics protocol is FS450_0002
|
| Scan protocol |
Arrays were scanned on a Affymetrix Gene Chip Scanner 3000 7G
|
| Data processing |
R/Bioconductor, normalized using GCRMA
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael Antosh |
| Organization name |
Michael Antosh
|
| Street address |
Brown University Box 1843
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE26724 |
Calorie/Dietary Restriction and Resveratrol in Female Drosophila Melanogaster Head/Thorax |
|
| Relations |
| Reanalyzed by |
GSE119084 |
