|
| Status |
Public on Nov 12, 2011 |
| Title |
Subject 35 Time 4 |
| Sample type |
RNA |
| |
|
| Source name |
vaginal epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: vaginal epithelium
dryness group: dry
weeks since first clinic visit: 4
|
| Treatment protocol |
Cytobrushes (Cooper Surgical) were used to collect vaginal samples from ~5cm into the vagina. These were suspended in 700ul RNAlater (Ambion).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol was used to extract total RNA from samples within 72 hours of collection following the manufacturer’s instructions. An extra step was added after homogenization and before addition of chloroform where excess un-homogenized material was cleared from the TRIzol by centrifugation at 12000g for 10 min.
|
| Label |
biotin
|
| Label protocol |
Labeling was carried out with the Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays and Affymetrix GeneChip WT Terminal Labeling kit as per the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridized for 16 hours at 45 C. All liquid handling steps were performed with GeneChip Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
Scanning done with a GeneChip Scanner 3000 7G and Command Console v1.1 (Affymetrix)
|
| Description |
E0448.CEL
|
| Data processing |
.CEL files were imported into Partel GS 6.4 using RMA processing.
|
| |
|
| Submission date |
Jan 20, 2011 |
| Last update date |
Nov 13, 2011 |
| Contact name |
Jordan Eduard Bisanz |
| E-mail(s) |
[email protected]
|
| Organization name |
Lawson Health Research Institute
|
| Street address |
268 Grosvenor Street, Room F2-116
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 4V2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE26761 |
Vaginal epithelial gene expression in postmenopausal women suffering from vaginal dryness |
|
