|
| Status |
Public on May 09, 2011 |
| Title |
E2-treated cells (3hr), biological rep_3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7 breast cancer cell line
agent: estradiol (E2)
time: 3hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized on U133A GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-2v4.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
|
| Description |
H6.3hrE2
MCF-7 cells were plated in 10cm dishes and then switched to SFM on the next day. Following overnight starvation, cells were treated in triplicate with E2 (10nM) for 3hr.
|
| Data processing |
log2 expression values estimated using GC RMA with Array analyzer software
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
May 09, 2011 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE26834 |
Expression data from MCF-7 cells stimulated by Estrogen or IGF-I |
|
