|
| Status |
Public on Jan 25, 2012 |
| Title |
HeLa cells 12h released, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa cells
growth condition: 12h released
|
| Growth protocol |
RNA was purified from HeLa cells in double thymidine (0) and cells released into the cell cycle after washing out the thymidine. RNA was collected from cells at 2, 4, 6, 8 and 12 hours after washing out the thymidine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. The RNA was then Dnase treated and purified using Qiagen Rneasy columns.
|
| Label |
biotin
|
| Label protocol |
RNA from each sample was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA.
|
| |
|
| Hybridization protocol |
The cRNA was hybridized to a microarray chip.
|
| Scan protocol |
The bound cRNA was stained with fluorescent streptavidin.
|
| Description |
HeLa_12hR_3
|
| Data processing |
R Bioconductor Affy package was used to analyze the microarray files. After loading the raw CEL files in R, RMA (Robust Multi-Array Averaging) was used to subtract background, normalize intensities and summarize gene expression levels
|
| |
|
| Submission date |
Jan 27, 2011 |
| Last update date |
Jan 25, 2012 |
| Contact name |
Subhashini Sadasivam |
| E-mail(s) |
[email protected]
|
| Organization name |
Dana Farber Cancer Insitute
|
| Department |
Medical Oncology
|
| Lab |
Laboratory of James DeCaprio, Mayer 444
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE26922 |
Cell cycle expression profiles in HeLa cells |
| GSE27031 |
The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression |
|
