|
| Status |
Public on Nov 09, 2011 |
| Title |
E. coli ZK126 and P. aeruginosa culture rep1 |
| Sample type |
RNA |
| |
|
| Source name |
E. coli and P. aer. Mixed culture
|
| Organism |
Escherichia coli |
| Characteristics |
strain: ZK126
culture: E. coli and P. aeruginosa culture
|
| Treatment protocol |
Following incubation samples were stabilized with RNA later (Ambion) and stored at 4C prior to RNA extraction
|
| Growth protocol |
E coli and P aeruginosa cells were grown aerobically at 37C in pure and mixed culture in LB broth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA extraction following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Enzo Bioarray Terminal Labeling Kit (Affymetrix cat # 900181) according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 4-6ug cDNA was hybridized to E coli or P aeruginosa gene chips
|
| Scan protocol |
Gene chips were scanned using an Affymetrix Model 3000 GeneArray scanner
|
| Description |
Gene expression data from mixed culture
|
| Data processing |
Microarray .cel files were analyzed by multiarray analysis method using the Affy package (version 1.18.2, Affymetrix) using default options.
|
| |
|
| Submission date |
Jan 28, 2011 |
| Last update date |
Nov 09, 2011 |
| Contact name |
Robert JC McLean |
| E-mail(s) |
[email protected]
|
| Phone |
512-245-3365
|
| Fax |
512-245-8713
|
| URL |
http://www.bio.txstate.edu/~micro/mclean/mclean.html
|
| Organization name |
Texas State University-San Marcos
|
| Department |
Biology
|
| Lab |
Supple 377
|
| Street address |
601 University Drive
|
| City |
San Marcos |
| State/province |
TX |
| ZIP/Postal code |
78666 |
| Country |
USA |
| |
|
| Platform ID |
GPL3154 |
| Series (1) |
| GSE26931 |
Mixed Culture Gene Expression of E. coli and Pseudomonas aeruginosa |
|
