|
| Status |
Public on Oct 17, 2022 |
| Title |
C956566- |
| Sample type |
RNA |
| |
|
| Source name |
normal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tongue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned by the Affymetrix Scanner 3000(Affymetrix).
|
| Description |
LncRNA expression data from LncRNA expression data from normal tissue
|
| Data processing |
Affymetrix GeneChip Command Console (version 4.0, Affymetrix) software was used to extract raw data. Next, Expression Console (version1.3.1, Affymetrix) software offered RMA normalization for both gene and exon level analysis. Then the gene expression analysis and alternative splice analysis proceeded separately.
|
| |
|
| Submission date |
Oct 14, 2022 |
| Last update date |
Oct 18, 2022 |
| Contact name |
WEI Cao |
| E-mail(s) |
[email protected]
|
| Phone |
135 2488 8984
|
| Organization name |
Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
|
| Street address |
Zhizaoju Road 639
|
| City |
Shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
| |
|
| Platform ID |
GPL23178 |
| Series (1) |
| GSE215767 |
Expression of LncRNA in oral squamous carcinoma |
|
