|
| Status |
Public on Mar 01, 2023 |
| Title |
ATCC2001 cells rep3 |
| Sample type |
SRA |
| |
|
| Source name |
yeast cells
|
| Organism |
Nakaseomyces glabratus CBS 138 |
| Characteristics |
tissue: yeast cells
cell line: ATCC2001
cell type: yeast cells
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the tissue using TRIzol Reagent and genomic DNA was removed using DNase I.
RNA-seq transcriptome library was prepared following TruSeq RNA sample preparation Kit from Illumina using 1μg of total RNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
A3
|
| Data processing |
The raw paired end reads were trimmed and quality controlled by SeqPrep and Sickle with default parameters.
Clean reads were separately aligned to reference genome with orientation mode using HISAT2 software.
The mapped reads of each sample were assembled by StringTie in a reference-based approach.
Assembly: Candida glabrata CBS 138
Supplementary files format and content: Excel files include RPKM values for each Sample
|
| |
|
| Submission date |
Nov 03, 2022 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Ke-Zhi Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
|
| Department |
Department of laboratory medicine
|
| Street address |
197 Ruijin 2nd Road
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL32820 |
| Series (1) |
| GSE217218 |
Effect of Cdc50 knockout on gene expression in Candida glabrata |
|
| Relations |
| BioSample |
SAMN31592264 |
| SRA |
SRX18157895 |
