|
| Status |
Public on Oct 26, 2011 |
| Title |
Egr1m24.2 |
| Sample type |
RNA |
| |
|
| Source name |
Cultures of primary fibroblasts from neonatal foreskin, Egr1, 24h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: neonatal foreskin
cell type: primary fibroblasts
treatment: Egr1
time: 24h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard protocol as recommended by Illumina.
|
| Label |
Cy3
|
| Label protocol |
cDNA was labeled using an Ambion labeling kit (Ambion).
|
| |
|
| Hybridization protocol |
Hybridization to Illumina Human Ref-8 version 2 Expression Microarray Chips (Illumina, San Diego, CA) using the standard protocolas recommended by Illumina.
|
| Scan protocol |
Standard protocol as recommended by Illumina.
|
| Description |
Primary fibroblasts infected by Egr1 mutated adenoviral recombinant and incubated for 24h.
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 1.5.9). It was vst transformed and rsn normalized.
|
| |
|
| Submission date |
Feb 08, 2011 |
| Last update date |
Oct 26, 2011 |
| Contact name |
Swati Bhattacharyya |
| E-mail(s) |
[email protected]
|
| Phone |
312 503 0565
|
| Organization name |
Northwestern University
|
| Department |
Medicine
|
| Lab |
Rheumatology
|
| Street address |
240 E Huron st, McGAW, M230
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE27165 |
A profibrotic gene expression program induced by Egr-1 in skin fibroblasts |
|
